This roster reflects the call up for EURO 2024,
announced by manager Roberto Martínez, on May 21, 2024.
| Player | Position | Club |
| Diogo Costa | Goalkeeper | FC Porto |
| José Sá | Goalkeeper | Wolverhampton |
| Rui Patrício | Goalkeeper | AS Roma |
| Pepe | Defender | FC Porto |
| Rúben Dias | Defender | Manchester City |
| António Silva | Defender | Benfica |
| Gonçalo Inácio | Defender | Sporting CP |
| Danilo Pereira | Defender | Paris Saint-Germain |
| Diogo Dalot | Defender | Manchester United |
| João Cancelo | Defender | FC Barcelona |
| Nélson Semedo | Defender | Wolverhampton |
| Nuno Mendes | Defender | Paris Saint-Germain |
| Bruno Fernandes | Midfielder | Manchester United |
| João Palhinha | Midfielder | Fulham FC |
| Vitinha | Midfielder | Paris Saint-Germain |
| Rúben Neves | Midfielder | Al Hilal SC |
| João Neves | Midfielder | Benfica |
| Bernardo Silva | Midfielder | Manchester City |
| Otávio* | Midfielder | Al Nassr FC |
| Matheus Nunes** | Midfielder | Manchester City |
| Cristiano Ronaldo (C) | Forward | Al Nassr FC |
| João Félix | Forward | FC Barcelona |
| Rafael Leão | Forward | AC Milan |
| Gonçalo Ramos | Forward | Paris Saint-Germain |
| Diogo Jota | Forward | Liverpool FC |
| Pedro Neto | Forward | Wolverhampton |
| Francisco Conceição | Forward | FC Porto |
* Otávio was originally called up by Roberto Martínez on May 21, 2024, but was removed from roster due to injury on June 3, 2024
** Matheus Nunes replaced Otávio on June 3, 2024